SubtiBank SubtiBank
Version comparison:

2018-02-12 19:13:242025-05-27 22:07:48

locus

BSU16120

BSU_16120

geneLength

2073

2076

essential

yes

quasi

outlinks

bsu

BSU16120

BSU_16120

Gene

Coordinates

1,683,661 → 1,685,736

1,683,661 1,685,736

Gene

Phenotypes of a mutant

essential [Pubmed|25092907], non-essential according to [Pubmed|28189581], but only one resistance cassette was possible

essential [Pubmed|25092907]

non-essential according to [Pubmed|28189581], but only one resistance cassette was possible

essential, deletion of [[gene|topA]] results in the immediate acquisition of suppressor mutations that result in overexpression of [[protein|ParE]]-[[protein|ParC]] [pubmed|30957856]

The protein

Catalyzed reaction/ biological activity

relaxation of negatively supercoiled DNA

relaxation of negatively supercoiled DNA

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)

The protein

Protein family

topo IA family (according to Swiss-Prot)

Type IA topoisomerase family (together with [[protein|TopB]])(according to UniProt)

The protein

Paralogous protein(s)

[[protein|TopB]]

[[this]]

Biological materials

Mutant

BKK16120 (Δ[[gene|topA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCCCCTCAAGA, downstream forward: _UP4_TAGCGGTGAGCAGGGTCTTG

BKK16120 (Δ[[gene|topA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCCCCTCAAGA, downstream forward: _UP4_TAGCGGTGAGCAGGGTCTTG

GP1963 (Δ[[gene|topA]]::aphA3 (citB-alsT)16) [pubmed|30957856]

Biological materials

Expression vector

pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

References

Reviews

6305585, 16936698

6305585, 16936698, 29177730

References

Original publications

17114254, 21710567, 25092907, 16600296, 27378778, 9722641, 28189581, 28414321, 21710567, 29242163, 26490962, 25232096, 23514156, 22923519, 10713132, 12167668

17114254, 21710567, 25092907, 16600296, 27378778, 9722641, 28189581, 28414321, 21710567, 29242163, 26490962, 25232096, 23514156, 22923519, 10713132, 12167668, 30222737, 30957856, 30478267, 32232337

Biological materials

Expression vectors

pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab